Cytiva Single Cell GenomiPhi™ DNA Amplification Kit

Amplify whole genome DNA from as little as a single cell. Cytiva Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

Includes: Single Cell GenomiPhi Reaction buffer 1 x 275µL, Single Cell GenomiPhi Enzyme mix 1 x 25µL, Single Cell GenomiPhi Amplification mix 1 x 25µL, Single Cell GenomiPhi DTT Solution 1 x 100µL, Single Cell GenomiPhi Lysis buffer 1 x 100µL and Single Cell GenomiPhi Neutralisation buffer 1 x 100µL

Product Code	Brand	No. of Reactions
15301652	Cytiva 29108107	25
15311652	Cytiva 29108039	100

Description

• For whole genome amplification of genomic DNA from as little as a single cell
• Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
• Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
• Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
• Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
• Reaction time: 2 to 4 hours
• Product average size: >10kb

Applications

For SNP arrays, Next Gen Sequencing, aCGH

Recommended Storage

Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C

Specifications

• Content And Storage: Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C.
• Final Product Type: illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
• Includes: Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL
• Size: >10kb

• Description: Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.
• For Use With (Application): Genomic DNA Amplification
• Product Type: illustra Single Cell GenomiPhi DNA Amplification Kit
• Test Time: 2 to 4 hours

Safety and Handling

Signal Word

• Danger

Hazard Category

• Skin corrosion/irritation Category 1
• Skin corrosion/irritation Category 1 B

Hazard Statement

• H314-Causes severe skin burns and eye damage.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : potassium hydroxide