Invitrogen™ AccuPrime™ Pfx DNA Polymerase

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.

Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO₄. Reagents are provided for 200 amplification reactions of 50μL each.

Product Code	Brand	No. of Reactions
10472482	Invitrogen™ 12344024	200 Reactions
10227242	Invitrogen™ 12344032	1000 Reactions

Description

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity. Platinum anti-Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure). Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone.

Benefits of AccuPrime Pfx DNA Polymerase

• Greater than 26 times higher fidelity than Taq DNA polymerase
• Amplification of fragments up to 12 kb
• Minimized PCR optimization

Applications

• Amplification of DNA from complex genomic, viral, and plasmid templates
• RT-PCR
• High-fidelity PCR
• Hot-start PCR

Notes

• AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.
• The 10X AccuPrime Pfx reaction mix contains MgSO₄, dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• Detection Method: Primer-probe
• Polymerase: AccuPrime Pfx DNA Polymerase
• Shipping Condition: Wet or Dry Ice
• Fidelity (vs. Taq): 26X
• Overhang: Blunt
• Size (Final Product): 12 kb or less

• GC-Rich PCR Performance: Low
• Reaction Speed: Standard
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Hot Start: Built-In Hot Start
• Reaction Format: Separate Components

For Research Use Only. Not for use in diagnostic procedures.