Invitrogen™ PureLink™ Pro 96 Genomic DNA Purification Kit

The PureLink™ Pro 96 Genomic DNA Purification Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.

Brand:  Invitrogen™ K182104A

Product Code. 10696835

Includes:

80mL PureLink™ Pro 96 Genomic Lysis/Binding Buffer, 70mL PureLink™ Pro 96 Genomic Digestion Buffer, 2 x 100mL PureLink™ Pro 96 Genomic Wash Buffer 1, 2 x 75mL PureLink™ Pro 96 Genomic Wash Buffer 2, 160mL PureLink™ Pro 96 Genomic Elution Buffer, 8mL RNase A (20mg/mL), 8mL PureLink™ Pro 96 Proteinase K (20mg/mL), 4 PureLink™ Genomic DNA Filter Plate, 2 x 6 96 Deep Well Blocks, 20/pack PureLink™ Foil Tape

Description

• Kit flexibility: One kit works with a variety of sample types and sizes
• Ultraclean gDNA: Excellent A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀ absorbance ratios, regardless of sample source
• Improved design: Optimized, semi-skirted, 96-well plates compatible with centrifugation and most benchtop and robotic vacuum manifolds

Multiple sample sources, one kit
The PureLink™ Pro 96 Genomic DNA Purification Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual. Examples of sample sources and amounts that can be used with the PureLink™ Pro Genomic DNA Purification Kit include mammalian cells and tissue, FFPE tissue, Oragene™-preserved saliva, mouse and rat tails, non-nucleated whole blood (human, mouse), nucleated whole blood (bird), blood spot on paper, bacterial cells, and yeast cells.

Improved plate design
The PureLink™ Pro 96 Genomic DNA Purification Kit provides optimized, semi-skirted, 96-well plates, for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. Sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation (at ∽2 x g), or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

Higher yields and purity
The PureLink™ Pro 96 Genomic DNA Purification Kit outperforms competitor products in both DNA yield and purity for multiple sample types, including blood. You are also able to get greater yields of higher-purity gDNA (determined by A₂₆₀/A₂₈₀ measurements) no matter what your sample type is; from bacteria to tissues to blood to cells. The enhanced PureLink™ Lysis and Wash Buffers help consistently deliver purified gDNA samples with absorbance reading ratios of ≅1.9 for both A₂₆₀/A₂₈₀ and A₂₆₀/A₂₃₀, indicating no contamination carryover of protein or guanidine.

Not High Throughput-Compatible (Manual), High Throughput-Compatible

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage:

  • 80 mL PureLink Pro 96 Genomic Lysis/Binding Buffer; room temperature
  • 70 mL PureLink Pro 96 Genomic Digestion Buffer; room temperature
  • 2 x 100 mL PureLink Pro 96 Genomic Wash Buffer 1; room temperature
  • 2 x 75 mL PureLink Pro 96 Genomic Wash Buffer 2; room temperature
  • 160 mL PureLink Pro 96 Genomic Elution Buffer; room temperature
  • 8 mL RNase A; room temperature
  • 8 mL PureLink Pro 96 Proteinase K; room temperature, or 4°C for long-term storage
  • 4 PureLink Genomic DNA Filter Plates; room temperature
  • 2 x 6 plates 96 Deep Well Blocks; room temperature
  • 20/pack PureLink Foil Tape; room temperature
  
  

• Sample Type: Bacteria, Cells, Dried Blood Spots, FFPE (Formalin-fixed paraffin-embedded), Mouse/Rat Tail, Saliva, Buccal Swab, Tissue, Whole Blood, Yeast
• Elution Volume: 50 to 200 μL
• For Use With (Equipment): Biomek™ FX, Eppendorf epMotion™, TECAN Freedom Evo™, TECAN Genesis™, Robotic Liquid Handling Systems
• High-throughput Compatibility: High-throughput Compatible
• Shipping Condition: Room Temperature
• Yield: 0.5 mg (Binding capacity)

• Isolation Technology: Silica Plate
• Scale: Mini
• Final Product Type: gDNA
• For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
• Quantity: 4 x 96 Preps
• Test Time: 45 min. (Including sample homogenization)
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Buccal Swab: 1
  Cells: ≤10⁶
  Dried Blood Spots: ≤5 punches
  FFPE: ≤8 sections of 5-15 μM thickness
  Mouse Tail: 1 cm
  Rat Tail: 0.5 cm
  Saliva (Oragene™ preserved): ≤200 μL
  Tissue: ≤25 mg
  Whole Blood: ≤200 μL
  Yeast: ≤10⁷ cells

For Research Use Only. Not for use in diagnostic procedures.