Learn More
Invitrogen™ Terminal Deoxynucleotidyl Transferase, recombinant
Performance and quality tested
Brand: Invitrogen™ 10533065
83.33 GBP valid until 2024-09-28
Use promo code "21626" to get your promotional price.
Alert:
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical. See MSDS.
Alert:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”21626” to get your promotional price
Includes: Vial of 5X buffer [500mM potassium cacodylate (pH 7.2), 10mM CoCl2 , 1mM DTT]
Description
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is DNA polymerase that catalyzes addition of deoxynucleotides to 3fi hydroxyl terminus of DNA.
- Source: Purified from baculovirus clone of calf thymus TdT
- Performance and Quality Testing: Endonuclease, 3' and 5' exodeoxyribonuclease, and levels of incorporation tested
- Unit Definition: One unit incorporates 1nmol dATP into acid-precipitable material in 1 hr. at 37°C, using d(pA)50 as primer
- Unit Reaction Conditions: 0.2M potassium cacodylate (pH 7.2), 10mM MgO4 C4 H6 , 1mM 2-mercaptoethanol, 0.5mg/mL BSA, 100 flM d(pA)50, 1mM [3H]dATP, and enzyme in 0.15mL for 1 h at 37°C
Cloning; Restriction Enzyme Cloning; Homopolymer tailing of vector and insert for cloning; Labeling oligonucleotides with biotin, 32P- or 35S-label; Apoptosis (TUNEL)
Order Info
Shipping Conditions: Approved for shipment on wet or dry ice
Specifications
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C. | |
Terminal Deoxynucleotidyl Transferase | |
500 U |
Approved for shipment on Wet or Dry Ice | |
5X Buffer, Reaction Buffer | |
TdT |
For Research Use Only. Not for use in diagnostic procedures.