Nidogen G2 Domain Monoclonal Antibody (JF4), Invitrogen™

Rat Monoclonal Antibody

Brand:  Invitrogen MA517017

Product Code. 15358520

Description

MA5-17017 recognizes native and denatured mouse and human nidogen, and blocks binding of nidogen to laminin. MA5-17017 has been successfully used in ELISA and Western blot applications. The MA5-17017 immunogen is a recombinant fragment corresponding to the G2 region of mouse nidogen produced in Fisher rat plus YB2/0 cells.

Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulfated glycoprotein that is widely distributed in basement membranes. It is tightly associated with laminin and also binds to collagen IV. Entactin probably has a role in cell-extracellular matrix interactions.

Specifications

• Antigen: Nidogen G2 Domain
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: tissue culture supernatant diluted in 2M tris, acetic acid with 0.1% sodium azide
• Gene Accession No.: P10493, P14543
• Gene Symbols: NID1
• Immunogen: Recombinant fragment corresponding to the G2 region of mouse nidogen produced in Fisher rat plus YB2/0 cells
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Inhibition Assays, Western Blot
• Clone: JF4
• Conjugate: Unconjugated
• Gene: NID1
• Gene Alias: A630025O17; enactin; Ent; Ent 2; entactin; entactin 1; Entactin 2; entactin-1; NID; NID 2; NID1; NID-1; Nid2; Nidogen (enactin); nidogen 1; Nidogen 2; Nidogen precursor (Entactin); nidogen-1
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 18073, 4811
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid