Invitrogen™ No-Stain™ Protein Labeling Reagent

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Brand:  Invitrogen™ A44449

Product Code. 16341358

Description

• Flexible use labeling format—Use with any protein gel of choice to perform total protein labeling of the membrane post-transfer. Or use it as a fast protein stain after electrophoresis of gels you do not intend to transfer.
• Easy to use protocol—Mix and incubate with the transferred membrane. The reaction time is 10 minutes. Gel staining is also a 10 minute reaction.
• Flexible visualization—Use a wide-range of imagers with UV, Green LED or Fluorescence (∼488 mm) light source, including the iBright FL1500 Imaging System, to capture an image.
• Accurate total protein normalization—Designed to provide accurate total protein normalization over a broad linear range for protein detection: 1–80 μg total protein loaded per well.
• Sensitive and stable signal—Protein bands are detected down to 20 ng and the signal is compatible with downstream antibody detection.
• Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions.

Specifications

• Product Type: Protein Labeling Reagent
• Description: The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
• No. of Reactions: A standard kit will label 40 mini gels or 40 mini gel-sized membranes
• Shipping Condition: Dry Ice
• Gel Compatibility: Any Gel Type and Gel Chemistry
• Product Line: Invitrogen™
• For Use With (Application): Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection

• Content And Storage: • No-Stain activator, 800 μL, store at -20°C
  • No-Stain derivatizer, 800 μL, store at -20°C
  • No-Stain labeling buffer (20X), 2 x 20 mL, store at 15°C to 30°C
• For Use With (Equipment): Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
• Run Time: 10 minutes
• Detection Method: Chemiluminescence, Fluorescence
• Membrane Compatibility: PVDF, Nitrocellulose
• Quantity: 40 reactions

Safety and Handling

Product Identifier

• 40RXN No-Stain Protein Labelling Reagent

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P370+P378-In case of fire: Use dry sand, dry chemical or alcohol resistant foam to extinguish.
• P403-Store in a well-ventilated place.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Mandelonitrile, DMSO

For Research Use Only. Not for use in diagnostic procedures.