Invitrogen™ Adiponectin Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ KMP0041

Product Code. 10068522

Includes: Pre-coated plate, standard, controls, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Mouse Adiponectinn ELISA quantitates Ms Adiponectin in mouse serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Adiponectin. Principle of the method The Mouse Adiponectin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid catabolism. Adiponectin is exclusively secreted from adipose tissue into the bloodstream and is very abundant in plasma relative to many hormones. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Adiponectin is secreted into the bloodsteam where it accounts for approximately 0.01% of all plasma protein. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels. Adiponectin automatically self-associates into larger structures. Adiponectin binds to a number of receptors. So far, two receptors have been identified, with homology to G protein-coupled receptors. Adiponectin exerts some of its weight reduction effects via the brain. This is similar to the action of leptin, but the two hormones perform complementary actions, and can have additive effects. Adiponectin affects glucose flux (gluconeogenesis, glucose uptake), lipid catabolism (beta-oxidation, triglyceride clearance), endothelial dysfunction, insulin sensitivity and weight loss. Administration of Adiponectin remains a promising area of research for clinical therapy in diseases such as obesity, type 2 diabetes and fatty liver disease.

Specifications

• Accession Number: Q60994
• Assay Sensitivity: 50 pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: 30kDa,APN,Acdc,Acrp30,Ad,GBP28,adipo,apM1
• Interassay CV: 5.2%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: Adiponectin
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 0.125-8 ng/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Colorimetric Detection
• Gene ID (Entrez): 11450
• Incubation Time: 2.3 hr.
• Intraassay CV: 2.6%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma, 10 μL; Serum, 10 μL; Supernatant, 10 μL
• Target: Mouse
• Total Assay Time: 3 hr. 20 min.

For Research Use Only. Not for use in diagnostic procedures.