Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media

Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

Brand:  Cytiva 17-5280-04

Product Code. 11379931

Description

• Low leakage of protein A with nearly twice total IgG binding capacity of Protein A Sepharose CL-4B
• Used in large-scale FDA-approved processes
• Manufactured without using animal-derived components
• Rigidity and high degree of substitution of Sepharose 4 Fast Flow matrix facilitates speedy process of large volumes dilute cell culture fluid
• High chemical, thermal and mechanical stability
• Withstands high concentrations of hydrogen bond disrupting agents

Specifications

• Quantity: 25 mL
• For Use With (Application): For The PurifiCatIon of Monoclonal and Polyclonal Antibodies at Both Laboratory and Process Scale
• Binding Capacity: >35 mg Human IgG/mL
• Flow Rate: 150 to 250 cm/Hoμr
• Height (English) Bed: 9.84 in.
• Max. Pressure: 100 kPa
• pH Range: 2 to 10
• Ligand Type: Native Protein A (S. aureus)

• Stability: No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol
• Storage Requirements: 2°C to 8°C
• Product Type: nProtein A Sepharose 4 Fast Flow Media
• Particle Size: 90 μm
• Height (Metric) Bed: 25 cm
• Matrix: 4% Cross-Linked Agarose
• Storage Buffer: 20% Ethanol

Safety and Handling

Product Identifier

• nProtein A Sepharose 4 Fast Flow, 25 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol