Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media

Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

Product Code	Brand	For Use With (Application)	pH Range	Quantity
11359931	Cytiva 17-5280-01	For PurifiCatIon of Monoclonal and Polyclonal Antibodies	3 to 9	5 mL
11379931	Cytiva 17-5280-04	For The PurifiCatIon of Monoclonal and Polyclonal Antibodies at Both Laboratory and Process Scale	2 to 10	25 mL

Description

• Low leakage of protein A with nearly twice total IgG binding capacity of Protein A Sepharose CL-4B
• Used in large-scale FDA-approved processes
• Manufactured without using animal-derived components
• Rigidity and high degree of substitution of Sepharose 4 Fast Flow matrix facilitates speedy process of large volumes dilute cell culture fluid
• High chemical, thermal and mechanical stability
• Withstands high concentrations of hydrogen bond disrupting agents

Specifications

• Stability: No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol
• Binding Capacity: >35 mg Human IgG/mL
• Flow Rate: 150 to 250 cm/Hoμr
• Height (English) Bed: 9.84 in.
• Max. Pressure: 100 kPa
• Storage Buffer: 20% Ethanol

• Storage Requirements: 2°C to 8°C
• Product Type: nProtein A Sepharose 4 Fast Flow Media
• Particle Size: 90 μm
• Height (Metric) Bed: 25 cm
• Matrix: 4% Cross-Linked Agarose
• Ligand Type: Native Protein A (S. aureus)