Macherey-Nagel™ Nucleospin™ 96 Plasmid Transfection-grade

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.

Brand:  Macherey-Nagel™ 740492.4

Product Code. 15553636

Description

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

• Purifies high-copy plasmids for transfection
• Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
• Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
• Ultra-fast procedure simplifies the work-flow
• High-throughput — 96-well plates allow for easy automation and supports plasmid screening
• Downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

• Bacterial Endotoxins: <50 EU/ng DNA
• Format: 96-well Plate
• Sample Type: Bacterial Culture (E. coli, High-copy Plasmids)
• Elution Volume: Elution Volume: 100 - 200 μL
• For Use With (Equipment): Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
• Includes: Filter Plates, Binding Plates, Buffers, RNase A
• Product Type: Core Kit
• Sample Volume: Up to 5 mL
• Test Time: 45 min/plate

• Content And Storage: Room temperature (18 - 25 °C)
• Isolation Technology: Silica Membrane and Endotoxin-reduction Technology
• Efficiency: Binding capacity: 20 μg
• Final Product Type: Transfection-grade plasmid DNA
• For Use With (Application): Rapid purification of plasmids for transfection
• No. of Samples: 4 x 96
• Purity: A₂₆₀/A₂₈₀: 1.80 - 1.85
• Size: < 15 kbp
• Yield: 5 - 20 μg

Safety and Handling

Product Identifier

• NucleoSpin 96 Plasmid T.-gr. Core (4x96)

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : sodium hydroxide, dodecyl sulphate, guanidine hydrochloride,triethanolamine, 2-propanol, RNAse