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Macherey-Nagel™ NucleoSpin™ miRNA Kit
Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.
Brand: Macherey-Nagel™ 740971.10
79.52 GBP valid until 2024-12-31
Use promo code "23283" to get your promotional price.
Code : X3
Additional Details : Weight : 0.06900kg Transport : UN number : 1993 Chem class : 3 Pack group : II
Description
- Parallel isolation of small and large RNA
- RNA purification fractionated by size:
- Isolation of small RNA only (< 200 b),
- Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
- Isolation of total RNA (small and large RNA in one fraction)
- Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
- Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
- NucleoSpin™ Filters for efficient sample homogenization
- rDNase for efficient on-column removal of genomic DNA
- Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue,
- and in combination with phenol / chloroform (e.g., TRIzol™) lysis
- Purification of siRNA and large dsRNA from DICER reactions
- Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization
Specifications
Mini Spin Column | |
NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase | |
NucleoSpin™ miRNA Kit | |
Small RNA: < 200 nt, large RNA: > 200 nt | |
10μg small RNA, 95μg large RNA |
Purification of siRNA and large dsRNA from DICER reactions | |
10 | |
Yes | |
<45 min./6 Reaction and <35 min./6 Reaction |
Safety and Handling
- Danger
Hazard Category
- Respiratory sensitiser Category 1
- Skin sensitiser Category 1
- Carcinogenicity Category 2
Supplemental information
- MIXTURE LIST-Contains : 1,4-dioxane, ethanol, guanidinium thiocyanate, 2-mercaptoethanol, rDNase