Macherey-Nagel™ NucleoSpin™ miRNA Kit

Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.

Product Code	Brand	No. of Reactions
11932512	Macherey-Nagel™ 740971.10	10
12788622	Macherey-Nagel™ 740971.250	250
12798622	Macherey-Nagel™ 740971.50	50

Description

Features

• Parallel isolation of small and large RNA
• RNA purification fractionated by size:
• Isolation of small RNA only (< 200 b),
• Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
• Isolation of total RNA (small and large RNA in one fraction)
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
• NucleoSpin™ Filters for efficient sample homogenization
• rDNase for efficient on-column removal of genomic DNA

Applications

• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue, and in combination with phenol / chloroform (e.g., TRIzol™) lysis
• Purification of siRNA and large dsRNA from DICER reactions
• Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization

Specifications

• Format: Mini Spin Column
• Includes: NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase
• Sample Size: Small RNA: < 200 nt, large RNA: > 200 nt
• Yield: 10μg small RNA, 95μg large RNA

• For Use With (Application): Purification of siRNA and large dsRNA from DICER reactions
• RNase: Yes
• Test Time: <45 min./6 Reaction and <35 min./6 Reaction