Macherey-Nagel™ NucleoSpin™ miRNA Kit

Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.

Brand:  Macherey-Nagel™ 740971.50

Product Code. 12798622

Description

Features

• Parallel isolation of small and large RNA
• RNA purification fractionated by size: 
• Isolation of small RNA only (< 200 b),
• Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
• Isolation of total RNA (small and large RNA in one fraction)
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform 
•   (patent pending)
• NucleoSpin™ Filters for efficient sample homogenization
• rDNase for efficient on-column removal of genomic DNA

Applications

• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue,
•    and in combination with phenol / chloroform (e.g., TRIzol™) lysis 
• Purification of siRNA and large dsRNA from DICER reactions
• Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization

Specifications

• Format: Mini Spin Column
• Includes: NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase
• Product Type: NucleoSpin™ miRNA Kit
• Sample Size: Small RNA: < 200 nt, large RNA: > 200 nt
• Yield: 10μg small RNA, 95μg large RNA

• For Use With (Application): Purification of siRNA and large dsRNA from DICER reactions
• No. of Reactions: 50
• RNase: Yes
• Test Time: <45 min./6 Reaction and <35 min./6 Reaction

Safety and Handling

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 2
• LONG-TERM AQUATIC HAZARD Chronic 3
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 2
• Flammable liquid Category 3
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H335-May cause respiratory irritation.
• H351-Suspected of causing cancer.
• H411-Toxic to aquatic life with long lasting effects.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .