Macherey-Nagel™ NucleoSpin™ miRNA Kit

Isolate small and large RNA in parallel. NucleoSpin™ miRNA Kits work on a wide varitey of tissue and cell culture formats.

Brand:  Macherey-Nagel™ 740971.10

Product Code. 11932512

Description

Features

• Parallel isolation of small and large RNA
• RNA purification fractionated by size: 
• Isolation of small RNA only (< 200 b),
• Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
• Isolation of total RNA (small and large RNA in one fraction)
• Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
• Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform (patent pending)
• NucleoSpin™ Filters for efficient sample homogenization
• rDNase for efficient on-column removal of genomic DNA

Applications

• Parallel isolation of small and large RNA from human / animal tissue and cultured cells, from plant tissue,
•    and in combination with phenol / chloroform (e.g., TRIzol™) lysis 
• Purification of siRNA and large dsRNA from DICER reactions
• Typical downstream applications: real-time RT-PCR, Northern blotting, chip hybridization

Specifications

• Format: Mini Spin Column
• Includes: NucleoSpin™ RNA Columns, NucleoSpin™ miRNA Columns, NucleoSpin™ Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin™ Filters, buffers, RNase-free rDNase
• Product Type: NucleoSpin™ miRNA Kit
• Sample Size: Small RNA: < 200 nt, large RNA: > 200 nt
• Yield: 10μg small RNA, 95μg large RNA

• For Use With (Application): Purification of siRNA and large dsRNA from DICER reactions
• No. of Reactions: 10
• RNase: Yes
• Test Time: <45 min./6 Reaction and <35 min./6 Reaction

Safety and Handling

Signal Word

• Danger

Hazard Category

• Respiratory sensitiser Category 1
• Skin sensitiser Category 1
• Carcinogenicity Category 2

Supplemental information

• MIXTURE LIST-Contains : 1,4-dioxane, ethanol, guanidinium thiocyanate, 2-mercaptoethanol, rDNase