Macherey-Nagel™ NucleoSpin™ RNA Virus 50 Preps

Mini spin kit for the isolation of viral RNA and DNA from cell-free biological fluids

Brand:  Macherey-Nagel™ 740956.50

Product Code. 12791021

Description

NucleoSpin™ RNA Virus is designed for the isolation of viral nucleic acids from serum, plasma or any cell-free biological fluids, featuring a special type of membrane which has a high binding capacity for nucleic acids.
The procedure is rapid and easy. The lysate is incubated for 5 to 10min and applied to the NucleoSpin™ column. After two washing steps to remove contaminations the RNA can be eluted with water or low-salt buffer. The kit contains all necessary buffers, spin columns and detailed protocols. Generally, NucleoSpin™ RNA Virus is suited for the parallel purification of viral RNA and DNA (support protocol). For example, isolation of HBV DNA is performed with an additional proteinase K digest (enzyme not included in the kit). For isolation of viral DNA with highest sensitivities, the NucleoSpin™ Blood kit is recommended.

Kit components: NucleoSpin™ RNA Virus columns, 2mL collecting tubes, buffers, RNase-free water, carrier RNA.
• Silica membrane technology
• Typical recovery: >90%
• Sample material: 150μL serum, plasma, cell-free biological fluids
• Fragment size: 100bp to 30kb
• Elution volume: 50μL
• Binding capacity: 40μg
• Preparation time: 30min/4-6 preps
• Format: mini spin column
• Support protocol for parallel isolation of viral RNA and DNA (proteinase K required)
• Applicable for HCV, HBV, HAV, HIV, HSV, HPV, VZV, EBV, parvovirus B19, H5N1

Specifications

• Content And Storage: 18°C to 25°C
• Sample Type: Cell-free Biological Fluids, Plasma, Serum
• No. of Reactions: 50 Preps
• Size: 100 bp−approx. 50 kbp

• Format: Mini Prep
• For Use With (Application): Isolation of Viral Nucleic Acids
• Sample Volume: < 150 L
• Target: Viral RNA and DNA

Safety and Handling

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P264-Wash with water thoroughly after handling.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.

Supplemental information

• MIXTURE LIST-Contains : guanidine hydrochloride, ethanol