Macherey-Nagel™ NucleoSpin™ TriPrep, Mini kit for RNA, DNA, and Protein Purification

Product Code. 11972392

Brand: Macherey-Nagel™ 740966.50

Description

Parallel isolation of RNA, DNA and, protein from an undivided sample.

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.

Superior selectivity - purify your RNA fractionated by size

Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)

Additional isolation of total protein

Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.

Excellent RNA recovery - even without phenol/chloroform

Lysis with chaotropic saltSpin column based procedure

Convenient handling - complete equipment provided

NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT

• Silica membrane technology
• Format: minispin columns
• Sample size: <10⁷ cultured cells, <30mg human/tissue; <50 mg plant tissue, <150μL reaction mixture
• Fragment size, small RNA: 18 to 200 bases
• Fragment size, large RNA: >200 bases
• Typical yield: 10μg small RNA; 95μg large RNA from 10⁷ HeLa cells
• Binding capacity: 200μg
• Elution volume, 30μL to 100μL
• Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
• Small RNA (18 to 200 bases)
• Large RNA (>200 bases)
• Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.

Spécification

• Filter Type: Shredders
• Product Type: NucleoSpin TriPrep
• Quantity: 1 Ea.
• Includes: With 2mL/1.5mL Collection Tubes, NucleoSpin Filters, buffers, RNase-free rDNase
• Column Type: Spin Column
• For Use With (Application): Rapid purification of total RNA, DNA, and protein from small and precious samples

Sécurité et traitement

Mention d'avertissement

• Danger

Catégorie de danger

• Flammable liquid Category 3
• Acute toxicity Category 4
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• LONG-TERM AQUATIC HAZARD Chronic 3

Mention de danger

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Conseils de prudence

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Informations supplémentaires

• MIXTURE LIST-Contains : zinc chloride; guanidinium thiocyanate,ethanol (denatured with 1%IPA/1%MEK, acc.2016/1867/EU); rDNa