Gibco™ Opti-MEM™ I Reduced Serum Medium

Opti-MEM™ I Reduced Serum Medium

Brand:  Gibco™ 31985054

Product Code. 12087549

Description

Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology. Opti-MEM™ I medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Opti-MEM™ I modifications for a range of cell culture applications. The complete formulation is confidential. For more information, please contact Technical Services.

Using Opti-MEM™ I Medium
Opti-MEM™ I Reduced-Serum Medium is a unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Opti-MEM™ I medium uses a sodium bicarbonate buffer system (2.4 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP manufacturing and quality system
Opti-MEM™ I is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Opti-MEM™ I product made in our Grand Island facility. This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standards.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Description: Opti-Mem™ Reduced-Serum Medium, 10 x 500mL, Liquid, 1 X Concentration, 7.0 to 7.4 pH, With L-Glutamine, Phenol Red, Sodium Pyruvate, 18 Months Shelf Life, 280 to 320mOsm/kg Osmolality, For Mammalian Cell Culture, Transfection
• Form: Liquid
• With Additives: Glutamine, Phenol Red, Sodium Pyruvate
• pH: 7.0 to 7.4

• Content And Storage: 2 to 8°C
• Concentration: 1 X
• Product Type: Reduced-Serum Medium
• For Use With (Application): Mammalian cell culture, transfection
• Quantity: 10 x 500 mL