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DiscoverX PathHunter™ eXpress FGR Activity Assay

Product Code. 17393949
Encompass
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Quantity:
1,000 dp (10 x 96-well)
Unit Size:
1 set
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Product Code. 17393949

Brand: DiscoverX 930884E3CP16L

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This item is not returnable. View return policy

Description

The PathHunter™ eXpress FGR Activity Assay measures FGR CTK activity via ligand-induced SH2 domain protein recruitment. eXpress kits contain all assay materials: cells, reagents, and plates.

The PathHunter™ eXpress FGR Activity Assay provides an easy-to-use cell based assay to study small molecule drug candidates targeting CTKs. This kit assesses CTK activation via detection of resulting GHR phosphorylation-induced SH2 domain protein recruitment, mediated by FGR-JAK2 fusion protein. This product uses EFC technology. The included cells overexpress PK-tagged relevant cytokine receptor and EA-tagged SH2 domain protein alongside an engineered fusion of the cytokine receptor binding domain of JAK2 (Janus Kinase 2) to the catalytic domain from the CTK of interest, FGR. Upon activation of the cytokine receptor, the CRBD-CTK fusion phosphorylates the receptor, leading to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Specifications

Assay Entry SH2 recruitment
For Use With (Application) Assay-Ready eXpress Kits
Primary Agonist Recombinant Human Growth Hormone
Quantity 1,000 dp (10 x 96-well)
Target Entry FGR
Cell Line U2OS
Target FGR
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