DiscoverX PathHunter™ eXpress TACR2 Activated GPCR Internalization Assay

The PathHunter™ eXpress TACR2 Activated GPCR Internalization Assay measures TACR2 (GPCR) internalization via early-endosome localization. eXpress kits contain all assay materials: cells, reagents, and plates.

Brand:  DiscoverX 93-0677E3CP0L

Product Code. 17323639

Description

The PathHunter™ TACR2 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit assesses ligand (e.g. tachykinin, substance P) based internalization of TACR2 via detection of localization to the early endosome. This product uses EFC technology. The included cells overexpress EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged TACR2. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting TACR2-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Specifications

• Assay Entry: GPCR
• Quantity: 1,000 dp (10 x 96-well)
• Target: TACR2

• For Use With (Application): Assay-Ready eXpress Kits
• Target Entry: TACR2