missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Phire Hot Start II DNA Polymerase

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.

Brand:  Thermo Scientific™ F122L

374.40 GBP valid until 2024-12-31
Use promo code "21651" to get your promotional price.


 View more versions of this product

Product Code. 10247467

  • £576.00 / 1000 reactions

Please to purchase this item. Need a web account? Register with us today!

Explore more special offers
This item is not returnable. View return policy

Description

Description

Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10–15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase (Cat. No. EP0072).

The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.

Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

Features

  • No reactivation step with hot start
  • Amplification four times faster than hot start Taq
  • Minimal reaction optimization due to high inhibitor tolerance
  • Abundant products due to high efficiency
  • Amplifies significantly longer DNA fragments than hot start Taq
  • Direct loading on gel with Green Buffer

Applications

  • Hot Start PCR
  • Standard PCR
  • Non-high-fidelity PCR
  • Fast PCR
  • High throughput PCR
  • Genotyping

Notes

  • The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

• Phire Hot Start II DNA Polymerase, 1 x 1 mL
• 5X Phire Reaction Buffer, 7 x 1.5 mL
• DMSO, 1 x 500 μL

Store at -20°C.
Phire Hot Start II DNA Polymerase
DNA Polymerase
1,000 reactions
Cloning, Hot-start PCR
Built-In Hot Start
Blunt
DNA
Medium
Fast
DHPLC
Wet or Dry Ice
2X
1000 Reactions
Separate Components
Colorless
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Special Offers

Special Offers

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.

For Research Use Only. Not for use in diagnostic procedures.