Invitrogen™ PichiaPink™ Expression Strain Set

The PichiaPink™ Expression Strain Set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Expression System Kits.

Brand:  Invitrogen™ A11154

Product Code. 10724144

Description

The PichiaPink™ Expression Strain Set contains the 4 Pichia pastoris strains for use with the PichiaPink™ Expression System Kits. Each strain comes as a frozen stock in YPD media with 15% glycerol. All PichiaPink™ strains have the ade2 deletion. 3 strains have deletions of two protease genes, PEP4 and PRB1. You can use the PichiaPink™ Expression Strain Set to refill your PichiaPink™ Yeast Expression System Kits.

The PichiaPink™ Expression Strain Set includes the following Pichia strains:

• PichiaPink™ Strain 1: ade2
• PichiaPink™ Strain 2: ade2, pep4
• PichiaPink™ Strain 3: ade2, prb1
• PichiaPink™ Strain 4: ade2, prb1, pep4

Select Transformants by Color

The PichiaPink™ Yeast Expression System makes it easy to select for transformed colonies by color. Untransformed PichiaPink™ strains form pink colonies. When grown on plates without adenine, transformed PichiaPink™ strains form white colonies (Figure 1).

Get Reduced Protein Degradation

The PichiaPink™ Expression Strain Set comes with 3 strains that include knockouts in two protease genes. Protease-deficient strains reduce the need for protease inhibitors when growing your cells and improve the yield of your protein. Test a single protease knockout or the double protease knockout to find the one that works best for you.

Why Choose the PichiaPink™ Yeast Expression System?

The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.

For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.

References

1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]

2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]

Specifications

• Bacterial or Yeast Strain: PichiaPink™
• Species: P. pastoris
• For Use With (Application): Yeast Expression
• High-throughput Compatibility: High-throughput Compatible
• Quantity: 4 x 1 mL

• Content And Storage: The PichiaPink™ Expression Strain Set consists of four glycerol strains (1 mL each). All four strains are protease mutants except for Strain 1.
  •PichiaPink™ Strain 1 (ade2)
  •PichiaPink™ Strain 2 (ade2, pep4)
  •PichiaPink™ Strain 3 (ade2, prb1)
  •PichiaPink™ Strain 4 (ade2, prb1, pep4)
  
  Store glycerol strains at -80°C.
• Format: Tube(s)
• Shipping Condition: Room Temperature
• Product Type: Expression Strain Set

For Research Use Only. Not for use in diagnostic procedures.