Thermo Scientific™ Pierce™ Cell Surface Protein Isolation Kit

Selectively biotinylate and purify mammalian cell surface proteins and exclude intracellular proteins with this complete set of reagents.

Brand:  Thermo Scientific™ 89881

Product Code. 10230104

Description

Thermo Scientific™ Cell Surface Protein Isolation Kit is a complete set of reagents for selective biotinylation and subsequent purification of mammalian cell surface proteins to the exclusion of intracellular proteins.

The kit efficiently labels proteins that have accessible lysine residues and sufficient extracellular exposure.The isolation procedure uses a cell-impermeable, cleavable biotinylation reagent (Sulfo-NHS-SS-Biotin) to label exposed primary amines of proteins on the surface of intact adherent or suspension cells. Treated cells are then harvested and lysed, and the labeled surface proteins are affinity-purified using Thermo Scientific NeutrAvidin Agarose Resin. The isolated cell surface proteins contain a small, nonreactive tag of the originally labeled primary amines but are no longer biotinylated (biotin remains bound to the resin). The kit contains sufficient reagents for eight experiments, each involving four T75 flasks of confluent cells.

Highlights:

• Isolates cell surface proteins – reduces complexity of total cellular protein

• Efficiently recovers labeled proteins – cleavable biotin allows for nearly 100% recovery of isolated cell surface proteins

• Convenience – all reagents are provided in one kit, along with complete instructions for labeling, cell lysis and purification of cell surface membrane proteins

• Western blotting applications – proteins recovered in SDS-PAGE buffer are loaded directly onto polyacrylamide gels

• Robust system – protocol designed for diverse cell lines, including NIH 3T3, HeLa, C6 and A431

Includes:

Biotinylation reagent, cell lysis reagent, agarose affinity resin, various buffers and microcentrifuge spin columns

Specifications

• Product Type: Protein Isolation Kit
• Form: Liquid, Powder
• Buffer: Lysis Buffer
• Sufficient For: 8 Reactions

• Content And Storage: Upon receipt store kit at 4°C. Product shipped at ambient temperature.
• Product Line: Pierce™
• Reagent Type: Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
• Quantity: 1 Kit

Safety and Handling

Product Identifier

• CELL SURFACE PROT ISOLATION KIT

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3
• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H319-Causes serious eye irritation.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H335-May cause respiratory irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P264-Wash thoroughly after handling.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P330-Rinse mouth.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

Supplemental information

• MIXTURE LIST-Contains : Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.