Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, WGA

Purify glycoproteins from serum and cell lysates using affinity resins or kits with concanavilin A (ConA) or wheat germ agglutinin (WGA) lectins.

Brand:  Thermo Scientific™ 89805

Product Code. 10290154

Description

Thermo Scientific™ Pierce Glycoprotein Isolation Kits use ConA and WGA lectin affinity resins and optimized buffers to efficiently isolate glycoproteins from serum, tissue and cultured cell lysates, thus enabling downstream analysis.

These two kits use lectins concanavilin A (ConA) and wheat germ agglutinin (WGA), respectively, as ligands to bind specific classes of glycoproteins from complex protein samples. ConA lectin recognizes alpha-linked mannose and terminal glucose residues, while WGA lectin selectively binds to N-Acetyl glucosamine (GlcNAc) groups and to sialic acid. The kits include ConA or WGA beaded agarose resin, optimized binding/wash and elution buffers, and easy-to-use microcentrifuge spin columns. The kits have been used to successfully isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling. Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems. Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes.

Highlights:

• High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers

• Fast – glycoprotein purification in less than one hour

• Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate

• Robust – lectin does not leach from resin when processing sample

• Convenient – complete kits include lectin resins and spin columns with all necessary reagents

• Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

Includes:

ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.

Specifications

• Description: Pierce Glycoprotein Isolation Kit, WGA
• Product Line: Pierce™
• Target: Glycoprotein
• Stationary Phase: Lectin
• Format: Kit, Microcentrifuge, Spin Column

• Content And Storage: Sufficient For: Isolating glycoproteins with strong affinity for WGA from 10 samples of up to 640 μL (1 to 1.5 mg total protein) each
  • WGA Lectin Resin, 1.1 mL
  • Binding/Wash Buffer (5X), 6.5 mL
  • Elution Buffer, 5 mL
  • Spin Columns, 10 columns
  • Collection Tubes, 20 tubes
  
  Store at 4°C.
• Quantity: 1 Kit
• Type: Glycoprotein Isolation Kit
• Sufficient For: 10 Reactions
• Column Type: Affinity, Agarose Resin

For Research Use Only. Not for use in diagnostic procedures.