Thermo Scientific™ Pierce™ Direct Magnetic IP/Co-IP Kit

Conjugate any antibody or protein to magnetic beads and immunoprecipitate (affinity purify) target protein complexes without IP-antibody interference.

Brand:  Thermo Scientific™ 88828

Product Code. 12106500

Description

The Thermo Scientific™ Pierce Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilize IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

Magnetic IP with NHS chemistry is convenient and allows for the immobilization of IP antibody from any species and subtype without the variations in coupling efficiency seen with Protein A and Protein G. The IP antibody is covalently attached so target proteins or co-IP protein complexes can be eluted and analyzed without antibody contamination. The kit contains an optimized protocol and all buffers and reagents necessary for antibody coupling and high yield IP or co-IP using either manual magnetic stands or automated platforms such as the Thermo Scientific KingFisher Instruments.

Highlights:

• Antibody-free IP antibody is irreversibly attached to the magnetic beads, resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen

• Fast complete antibody conjugation and immunoprecipitation in less than 4 hours

• Convenient the IP/co-IP kit contains magnetic beads and all essential buffers for optimal immunoprecipitation

• Antibody compatible use with any antibody species, class or isotype

• Low nonspecific binding the bead surface is pre-blocked and all nonreacted NHS-ester groups are fully quenched

• Protocol-compatible protein coupling to the beads and immunoprecipitation can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments)

• Scalable use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody magnetic beads for multiple experiments

Includes:

NHS-Activated Magnetic Beads, IP lysis/wash buffer, elution buffer, neutralization buffer, quenching buffer, borate buffer and sample loading buffer

Specifications

• Content And Storage: Sufficient For: 40 IP reactions using 25 μL of beads
  • Pierce NHS-Activated Magnetic Beads, 1 mL
  • IP Lysis/Wash Buffer, 2 x 50 mL
  • Elution buffer, pH 2.0, 5 mL
  • Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL
  • Neutralization Buffer, pH 8.5, 0.5 mL
  • 0.67M Borate Buffer, 1 mL
  • BupH Borate Buffer Pack, 1 pack
  • Quenching Buffer, 25 mL
  
  Store at 4°C.
• For Use With (Equipment): KingFisher™ Flex, Magnetic Stand
• Quantity: 40 reactions
• Target: Not Target-Specific
• Sufficient For: 40 Reactions

• Assay: Affinity Purification
• Product Line: Pierce™
• For Use With (Application): Pull-Down, Immunoprecipitation (IP)
• Type: Immunoprecipitation/Co-Immunoprecipitation Kit
• Format: Kit

Safety and Handling

Product Identifier

• Pierce Direct Magnetic IP/Co-IP Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Reproductive toxicity Category 1B
• Skin corrosion/irritation Category 1 B
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H312-Harmful in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H331-Toxic if inhaled.
• H335-May cause respiratory irritation.
• H360FD-May damage fertility. May damage the unborn child.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P264-Wash hands thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P310-Immediately call a POISON CENTER/doctor/.
• P311-Call a POISON CENTER/doctor/ .
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P331-Do NOT induce vomiting.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P363-Wash contaminated clothing before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Boric acid, Sodium tetraborate decahydrate, Hydrogen chloride, Dimethylacetamide, Sodium chloride, T

For Research Use Only. Not for use in diagnostic procedures.