Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Brand:  Thermo Scientific™ 22582

Product Code. 10086113

Description

Thermo Scientific™ Pierce DTNB (Ellman's Reagent) reacts with sulfhydryl groups to yield a colored product, which provides a reliable method for measure free reduced cysteines and other free sulfhydryls in solution.

A solution of this compound produces a measurable yellow-colored product when it reacts with sulfhydryls. Consequently, Ellman's Reagent is very useful as a sulfhydryl assay reagent because of its specificity for –SH groups at neutral pH, high molar extinction coefficient and short reaction time. Sulfhydryl groups may be estimated in a sample by comparison to a standard curve composed of known concentrations of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups may be quantitated by reference to the extinction coefficient of TNB.

Highlights:

• Sulfhydryl detection– reacts quantitatively with free (reduced) sulfhydryl groups

• Colorimetric–yields colored product to enable spectrophotemetric measurement in tubes or microplates

• Proven assay method– well-characterized chemistry enables quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard

Specifications

• Product Type: DTNB
• Quantity: 5 g

Safety and Handling

Product Identifier

• DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.

Precautionary Statement

• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P310-Immediately call a POISON CENTER/doctor/.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 3,3'-dithiobis [6-nitrobenzoic] acid

For Research Use Only. Not for use in diagnostic procedures.