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Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.
Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).
Form | Solid |
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Short (2.8A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).
Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.
Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.
Product Type | Alkylating Reagent |
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Product Line | Pierce™ |
Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.
Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.
Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).
Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.
CAS | 9001-73-4 |
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Synonym | EC 3.4.22.2 |