Thermo Scientific™ Trypsin, TPCK Treated

Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.

Brand:  Thermo Scientific™ 20233

Product Code. 11896734

Description

Thermo Scientific™ Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.

Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.

Features of Thermo Scientific Pierce TPCK Trypsin
• Cleaves at carboxyl side of arginine and lysine residues
• Digestion conditions: pH 7.5–9.0, 37°C

Trypsin has a wide range of applications including amino acid analysis and protein sequencing, mapping and structural studies. Enzymes such as trypsin and chymotrypsin have become important tools in sequencing studies since they are highly selective in their cleavage of peptide bonds. Trypsin cleaves only those peptide bonds in which the carboxyl group is contributed by a lysine or an arginine residue, regardless of the length or amino acid sequence of the chain. Our Immobilized TPCK Trypsin can be substituted for free trypsin in many application and is advantageous because it minimizes autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the trypsin. Immobilized trypsin is also more stable against heat-induced denaturation, resulting in longer maintenance of activity.

Applications:
• Removal of adherent cells from tissue culture flasks
• Preparing tryptic fragments for Edman degradation sequencing
• Immobilized trypsin can be used to purify soybean trypsin inhibitor
• Use our Pierce Trypsin, MS Grade (Cat No. 90057) for mass spectrometry workflows

Trypsin is a 23.8kDa pancreatic serine endoprotease derived from trypsinogen, an inactive precursor zymogen, after enzymatic removal of an n-terminal leader sequence by enterokinase. Once some trypsin has been formed it can catalyze the conversion of more trypsinogen into its catalytically-active form. Trypsin is treated with L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotrypsin activity without affecting trypsin activity.

Dissolving 0.25 g of trypsin in 100 mL of chilled HBSS for a 0.25% w/v solution is recommended. Mix solution until homogenous and pH is 7.4 to 7.8. Filter the solution through a low protein-binding filter such as the Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units with PES Membranes (Cat No. 567-0010) and store -5°C to -20°C.

Related Products
Immobilized Trypsin, TPCK Treated (Agarose Resin) (Cat. No. 20230)

Specifications

• Description: Pierce TPCK Trypsin
• Form: Powder
• Product Type: Trypsin

• Content And Storage: Store in original container protected from direct sunlight in a dry, cool, and well-ventilated area from 2–8°C.
• Quantity: 50 mg

Safety and Handling

Product Identifier

• Trypsin, TPCK Treated

Signal Word

• Danger

Hazard Category

• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H335-May cause respiratory irritation.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Trypsin

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For Research Use Only. Not for use in diagnostic procedures.