Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.

Brand:  Thermo Scientific™ A39255

Product Code. 15976082

Description

Thermo Scientific™ Pierce DTT (Dithiothreitol) is offered as 7.7 mg x 48-tube microplates for convenient preparation of 100 μL of 0.5 M stocks for addition to protein samples for effective reduction of disulfide bonds.

Thermo Scientific Pierce DTT (Dithiothreitol), No-Weigh format, is offered as 7.7 mg x 48-tube microplates for convenient preparation of 100 μL of 0.5 M single-use stocks for addition to protein samples for effective reduction of disulfide bonds.

DTT is a popular reducing agent for many protein research applications, including preparing proteins for reducing polyacrylamide electrophoresis (SDS-PAGE) and reducing disulfide bonds for labeling sulfhydryl groups in conjugation reactions. At low concentrations, DTT is used to stabilize proteins and enzymes with free sulfhydryl groups to keep monothiols in a reduced state. DTT reduction is also used to prepare proteins for mass spectrometry.

Properties of DTT:

• Synonyms—1,4-dithiothreitol; DTT; Cleland’s Reagent; 1,4-dimercapto-2,3-butanediol
• Molecular weight (MW)—154.24
• Molecular formula—C₄H₁₀O₂S₂
• CAS number—3483-12-3

DTT is not stable in solution and should be prepared fresh for each use. The No-Weigh format consists of a 48-microtube tray containing dry, stable, single-use aliquots of the DTT chemical to eliminate the need to weigh small amounts of reagent and prevent waste of unused DTT. Just puncture the seal of one microtube, add 100 μL of water or buffer, and mix to dissolve. In just a few seconds you will have a solution of 500 mM DTT, ready for a variety of laboratory methods. Dilute as necessary or add the appropriate volume of the 500 mM stock solution to achieve the amount of DTT reducing agent required for your specific application.

Features of the No-Weigh format:

• Saves time—just add 100 μL water or buffer to make 500 mM DTT solution in seconds
• Eliminates waste—makes 100 μL at a time, just the right amount for most applications
• Always fresh—ensures full reducing strength for every use; no need to store stock solutions

With the convenient No-Weigh format, a 500 mM DTT solution can be made in less than 30 seconds. The unique packaging ensures that the DTT reducing agent is at full strength and able to protect proteins from oxidative damage or reduce any disulfides prior to electrophoresis.

Specifications

• Quantity: 48 x 7.7 mg
• Content And Storage: Upon receipt, store product at 4°C.
  
  
• Product Line: Pierce™

Safety and Handling

Product Identifier

• Pierce DTT, No-Weigh Forma

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.

Supplemental information

• MIXTURE LIST-Contains : 1,4-dimercapto-2,3-Butanediol

For Research Use Only. Not for use in diagnostic procedures.