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Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Brand:  Thermo Scientific™ 32134


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Product Code. 11547910

  • £138.00 / 25mL

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Description

Description

Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.

Highlights:

  • Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

  • Higher sensitivity — detect targets down to the low-picogram level

  • Longer signal duration — sustained light output for as long as five hours

  • More imaging options — X-ray, CCD or laser-based imagers

  • More affordable — high quality and performance at a lower price than other suppliers« similar substrates

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Substrate
Liquid
Nitrocellulose, PVDF
25 mL Kit
2°: 1:25-200K (5-40ng/mL), 1°: 1:1K (0.05- 1μg/mL)
5 hr.
Upon receipt store at 4°C. Product shipped on ice.
Chemiluminescence, Fluorescence
Western Blot
Pierce™
Horseradish Peroxidase
Low picogram to mid-femtogram
250 sq. cm of membrane
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Safety and Handling

Safety and Handling

Product Identifier
  • Pierce ECL Plus Western Blotting Substrate

Signal Word
  • Danger

Hazard Category
  • Carcinogenicity Category 2
  • Serious eye damage/eye irritation Category 2
  • Flammable liquid Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
  • H351-Suspected of causing cancer.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Ethanol

SDS
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