Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Product Code	Brand	Quantity	Sufficient For
11547910	Thermo Scientific™ 32134	25 mL Kit	250 sq. cm of membrane
11527271	Thermo Scientific™ 32132X3	300 mL Kit	3000 sq. cm of membrane
11517271	Thermo Scientific™ 32132	100 mL Kit	10 mini-gel blots; 1000cm2 of membrane

Description

Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.

Highlights:

• Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

• Higher sensitivity — detect targets down to the low-picogram level

• Longer signal duration — sustained light output for as long as five hours

• More imaging options — X-ray, CCD or laser-based imagers

• More affordable — high quality and performance at a lower price than other suppliers« similar substrates

Specifications

• Product Type: Substrate
• Form: Liquid
• Product Line: Pierce™

• Detection Method: Chemiluminescence, Fluorescence
• Membrane Compatibility: Nitrocellulose, PVDF
• Sensitivity: Low picogram to mid-femtogram

Safety and Handling

Product Identifier

• Pierce ECL Plus Western Blotting Substrate

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H351-Suspected of causing cancer.

Precautionary Statement

• P201-Obtain special instructions before use.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Ethanol

For Research Use Only. Not for use in diagnostic procedures.