Thermo Scientific™ Pierce™ Hydroxylamine-HCl

NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.

Brand:  Thermo Scientific™ 26103

Product Code. 11881405

Description

Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, including to cleave Asn-Gly peptide bonds and to deprotect (expose) sulfhydryls for conjugation following modification with SATA and similar reagents.

Thermo Scientific™ Pierce Hydroxylamine-HCl has many uses, which include cleaving Asn-Gly peptide bonds and deprotecting (exposing) sulfhydryls on SATA-modified molecules for conjugation. Hydroxylamine·HCl is a strong reducing agent that is useful in biochemical crosslinking applications, including the deacetylation of SATA and chemical cleavage of EGS and Sulfo-EGS. Hydroxylamine converts carbonyl compounds (aldehydes and ketones) to their oxime derivatives in the presence of a weak base. Therefore, crosslinkers and other compounds that contain a carbonyl within their structure are cleavable with hydroxylamine-HCl.

Highlights:

• Exposes protected sulfhydryl groups on SATA-modified molecules
• Cleaves carbonyl-containing crosslinkers such as EGS

Reagent Properties:

• Chemical name: Hydroxylamine Hydrochloride
• Chemical formula: NH₂OH·HCl
• Molecular weight: 69.49
• CAS Number: 5470-11-1
• Form: White crystals, free of foreign material
• Solubility: 10%

Specifications

• CAS: 5470-11-1
• Product Line: Pierce™
• Reagent Type: SATA
• Product Type: Hydroxylamine Hydrochloride

• Content And Storage: Upon receipt store desiccated at room temperature. Product shipped at ambient temperature.
• Form: Solid
• Quantity: 25 g

Safety and Handling

Product Identifier

• Hydroxylamine Hydrochloride 25 Gm

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• ACUTE AQUATIC Acute 1
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Substances and mixtures corrosive to metals Category 1
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1
• Specific target organ toxicity after repeated exposure Category 2

Hazard Statement

• H290-May be corrosive to metals.
• H301-Toxic if swallowed.
• H312-Harmful in contact with skin.
• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H351-Suspected of causing cancer.
• H373-May cause damage to organs.
• H400-Very toxic to aquatic life.

Precautionary Statement

• P201-Obtain special instructions before use.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P390-Absorb spillage to prevent material damage.
• P406-Store in corrosive resistant/ container with a resistant inner liner.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : Hydroxylamine, hydrochloride

For Research Use Only. Not for use in diagnostic procedures.