Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit

Easily capture and affinity purify binding partners of His-tagged fusion proteins using nickel-chelate (Ni-IDA) agarose beads and this complete reagent set.

Brand:  Thermo Scientific™ 21277

Product Code. 10351214

Description

Thermo Scientific™ Pierce PolyHis Protein Interaction Pull-Down Kit purifies protein interaction complexes involving practically any user-supplied His-tagged fusion protein.

The PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag. You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, cobalt-IMAC affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol. This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers.

Highlights:

• 6xHis pull-down – purifies protein interactors of any His-tagged fusion protein

• Complete kit – provides all components and detailed protocol for purifying protein:protein interactions

• No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge)

• Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of cobalt IMAC agarose beads, including simple processing of multiple samples

• Flexible – instructions include protocols for bait and prey proteins from different sources

Includes:

Cobalt chelate agarose resin, lysis buffer, TBS pack, imidazole solution for elution, microcentrifuge spin columns and collection tubes

Recommended for:

Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates

Specifications

• Content And Storage: Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
  • Cobalt Chelate Agarose Resin, 750 μL
  • Pull-Down Lysis Buffer, 250 mL
  • Imidazole Solution (4M), 5 mL
  • TBS Pack (makes 500 mL), 1 pack
  • Pierce Spin Columns and Accessories, 27 columns
  • Microcentrifuge Collection Tubes, 100 tubes
  
  Upon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
• For Use With (Equipment): Microcentrifuge
• Quantity: 1 Kit
• Target: Not Target-Specific
• Sufficient For: 25 Reactions

• Assay: Affinity Purification
• Product Line: Pierce™
• For Use With (Application): Pull-Down (Tag-Based)
• Type: Pull-Down Kit
• Format: Kit

Safety and Handling

Product Identifier

• Pierce His Protein Interaction Pull-Down Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 1
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Reproductive toxicity Category 1B
• Skin corrosion/irritation Category 1 B

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H314-Causes severe skin burns and eye damage.
• H318-Causes serious eye damage.
• H319-Causes serious eye irritation.
• H360-May damage fertility or the unborn child.

Precautionary Statement

• P201-Obtain special instructions before use.
• P202-Do not handle until all safety precautions have been read and understood.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P310-Immediately call a POISON CENTER/doctor/.
• P330-Rinse mouth.
• P331-Do NOT induce vomiting.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P363-Wash contaminated clothing before reuse.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 1-Imidazole, ethanol, Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.