Thermo Scientific™ Pierce™ Protein Refolding Kit

Refold recombinant proteins that have been denatured and solubilized from inclusion bodies by determining optimal buffer conditions using this reagent matrix strategy.

Brand:  Thermo Scientific™ 89867

Product Code. 10188053

Description

Thermo Scientific™ Pierce Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilized from inclusion bodies.

The kit contains the essential reagents and complete strategy for determining optimal buffer conditions to refold denatured recombinant proteins to restore native structure and function. Nine base refolding buffers form a matrix that includes a range of strong and weak denaturant conditions for the suppression of protein aggregation. The supplied additives are used as additional matrix factors, depending on the protein type being refolded. Buffer components are examined at three concentration levels, allowing a wide spectrum of folding conditions to be tested within one experiment. The adjustable design allows matrix conditions to be tailored to the target protein, preventing sample waste and unnecessary analysis, while maximizing refolding yields. The Pierce Protein Refolding Kit is accompanied by a comprehensive Refolding Guide with details on isolating, solubilizing and purifying inclusion bodies; optimizing refolding conditions; and analyzing refolding yields.

Highlights:

• Robust – conditions and components examined are limited to those having the most significant and general utility as folding buffers

• Convenient – three-level matrix design significantly reduces the amount of secondary optimization required and increases the ease of data interpretation

• Adjustable matrix format – allows refolding experiments to be customized to the target protein; known positive and negative interactions between buffer components are addressed, minimizing unnecessary analyses

• High-purity reagents – reagents are formulated using stringent standards so that consistent results are attained

Includes:

Nine different Base Refolding Buffers (10mL each), convenient amounts of seven additives, and a comprehensive Refolding Guidebook

Compatible with:

Thermo Scientific Inclusion Body Solubilization Reagent (Part No. 78115)

Specifications

• Product Type: Protein Refolding Kit
• Form: Liquid, Powder
• Reagent Type: Chaotrope, Denaturant

• Content And Storage: Upon receipt store at -20°C. Product may be stored at 4°C for up to two months. Product is shipped on dry ice.
• Product Line: Pierce™
• Quantity: 100 Reactions

Safety and Handling

Product Identifier

• Pierce Protein Refolding Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P271-Use only outdoors or in a well-ventilated area.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P330-Rinse mouth.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : (R*,R*)-1,4-dimercapto-2,3-butanediol, Sodium chloride

For Research Use Only. Not for use in diagnostic procedures.