Thermo Scientific™ Pierce™ Quantitative Peroxide Assay Kit (Aqueous

Detect and measure hydrogen peroxide levels (H2O2) in biological samples using an iron (Fe) and xylenol orange (XO) reagent for microplates or tubes.

Brand:  Thermo Scientific™ 23280

Product Code. 10414195

Description

Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits.

These aqueous and lipid-compatible peroxide assay kits detect peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. One assay kit has an aqueous-compatible formulation that includes sorbitol, which provides sensitivity enhancement. The other assay kit has a formulation that may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

In these assays, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Highlights:

• Peroxides – detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples

• Convenient – no proprietary reagents involved but the ready-to-use kits remove the variability, difficulty and added expense of formulating the reagents yourself

• Versatile – quantitation by calculation from the extinction coefficient or by reference to a standard curve enables the method to be easily adapted to a variety of sample types and testing purposes

• Two formulations – choose the aqueous-compatible kit for maximum sensitivity with non-lipid samples; choose the lipid-compatible kit for best results with lipid-containing samples

Recommended for:

General hydrogen peroxide (H²O²) measurement in protein samples; Quantitation of peroxides in detergents; Assessment of the effects of peroxides on cellular activity; Determination of protein glycation; Measurement of peroxide accumulation in lipids

Specifications

• Content And Storage: Sufficient For: 100 tube assays or 500 microplate assays with non-lipid samples
  • Reagent A, 1 mL
  • Reagent B, 2 x 50 mL
  
  Store at 4°C.
• Assay: Quantitative Peroxide Assay
• Product Line: Pierce™
• Specificity: Not Target-Specific
• For Use With (Application): Solution-based Detection, Absorbance

• Description: Pierce Quantitative Peroxide Assay Kit (Aqueous)
• Detection Method: Colorimetric
• Product Type: Quantitative Peroxide Assay
• Sufficient For: 100 Tube Assays or 500 Microplate Assays
• For Use With (Equipment): Spectrophotometer, Microplate Reader

Safety and Handling

Product Identifier

• Quantitative Peroxide Assay Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Flammable liquid Category 2
• Specific target organ toxicity after single exposure Category 1

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H301-Toxic if swallowed.
• H311-Toxic in contact with skin.
• H331-Toxic if inhaled.
• H370-Causes damage to organs.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
• P403+P233-Store in a well-ventilated place. Keep container tightly closed.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : methanol

For Research Use Only. Not for use in diagnostic procedures.