Thermo Scientific™ Rapid ELISA Mouse mAb Isotyping Kit

Isotype mouse monoclonal antibody using an antigen-independent, microplate-based method that enables multiple samples to be tested in about one hour.

Brand:  Thermo Scientific™ 37503

Product Code. 11814524

Description

Thermo Scientific™ Pierce Rapid ELISA Mouse Antibody Isotyping Kit provides antigen-independent determination of mouse monoclonal antibody isotype using a microplate method that enables multiple samples to be tested in about one hour.

The kit enables easy identification of mouse immunoglobulin class, subclass and light chain composition. This fast assay uses ELISA strip-plates pre-coated in different wells with anti-mouse heavy-chain capture antibodies (anti-IgG1, IgG2a, IgG2b, IgG3, IgA and IgM) or anti-mouse light-chain capture antibody (kappa or lambda). This approach eliminates the need to purify and immobilize an antigen to determine immunoglobulin isotype. A mouse monoclonal antibody sample applied to the wells can be isotyped within one hour. Results are evaluated qualitatively by visual inspection or quantitatively by measuring the absorbance at 450nm.

Highlights:

• Fast – determine antibody isotype in approx. 1 hour; considerably faster than traditional ELISA kits

• Convenient – eight-well strip format allows partial use of the plate; use one strip (column) for each sample (12 samples per plate)

• Specific – characterize antibodies for six different subclasses and two different light-chain types

• Sensitive – accurate characterization with samples containing more than 3ng/mL of test antibody

• Flexible – kit is compatible with hybridoma cell culture supernatant, ascites fluid or purified antibodies

• Cost effective – cost per assay is less than with single-use assay strips or cassettes

• No special equipment needed – assess results visually or measure quantitatively using an ordinary ELISA plate reader (450nm)

• Complete and easy to use – includes pre-coated plates, detecting antibody, buffers, and TMB substrate and stop solutions

Includes:

Precoated 96-well Isotyping plates, 5 plates; Goat anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30mL; TMB Substrate, 55mL; Stop solution, 55mL; 30X Wash buffer, 200mL; Tris buffered saline, 2 packs

Specifications

• Content And Storage: Sufficient For: 60 isotype determinations (8 wells per test sample)
  • Pre-coated 96-well Isotyping Plates, 5 plates
  • Goat Anti-Mouse IgG+IgA+IgM-HRP Conjugate, 30 mL
  • TMB Substrate, 55 mL
  • Stop Solution, 55 mL
  • 30X Wash Buffer, 200 mL
  • TBS Buffer Packs (each makes 500 mL), 2 packs
  
  Store at 4°C.
• For Use With (Equipment): Microplate Reader
• Assay: Isotyping Assay
• Product Line: Pierce™

• For Use With (Application): ELISA (Antigen Capture), Absorbance
• Quantity: 60 Tests
• Detection Method: Colorimetric
• Target Specificity: Target-specific

Safety and Handling

Product Identifier

• Rapid ELISA Mouse mAb Isotyping Kit

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Skin sensitiser Category 1

Hazard Statement

• H315-Causes skin irritation.
• H317-May cause an allergic skin reaction.
• H319-Causes serious eye irritation.
• H332-Harmful if inhaled.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.
• P362+P364-Take off contaminated clothing and wash it before reuse.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : 5-chloro-2-methyl-3(2H)-isothiazolone, Sodium chloride, Tris(hydroxymethyl) aminomethane, sulphuric

For Research Use Only. Not for use in diagnostic procedures.