Thermo Scientific™ Pierce™ TiO² Magnetic Phosphopeptide Enrichment Kit

Purify phosphorylated peptides from complex biological samples using titanium dioxide-coated magnetic beads; ideal for mass spectrometry preparation.

Brand:  Thermo Scientific™ 88811

Product Code. 10415335

Description

Thermo Scientific™ Pierce Titanium Dioxide Magnetic Phosphopeptide Enrichment Kit is for isolating phosphopeptides from complex biological samples using titanium dioxide-coated magnetic beads.

The Magnetic Phosphopeptide Enrichment Kit is for isolating phosphopeptides from complex biological samples using titanium dioxide-coated magnetic beads. The titanium dioxide (TiO₂) ligand on these magnetic particles selectively binds peptides containing phosphorylated serine (Ser), tyrosine (Tyr) or threonine (Thr), enabling selective phosphopeptide enrichment from protease-digested samples. The isolated phosphopeptides are compatible for analysis downstream by mass spectrometry. The binding, wash and elution buffers supplied in the kits ensure that maximum specificity and yield.

The high-performance iron oxide superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher™ 96 and KingFisher Flex Instruments. The beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.

Highlights:

• Complete MS-compatible kits – include ready-to-use binding, wash and elution buffers that are optimized for phosphopeptide enrichment and downstream analysis by MALDI and ESI mass spectrometry

• Optimized for HTS – procedure validated for processing 1 to 96 samples at a time; complete entire assay in about 15 minutes using a KingFisher Flex Instrument

• Stable affinity ligand – titanium dioxide is specially coated as a film on the magnetic particles

• Selective – affinity system is selective for phosphorylated Ser, Tyr and Thr; exhibits minimal non-specific binding to acidic residues

• Sensitive – affinity provides more than 1000 times greater sensitivity than traditional IMAC technologies; enables enrichment and MS-measurement of less than 100 fmol of phosphoprotein

Includes:

Pierce TiO² Magnetic Beads; Binding, wash and elution buffers; 96-well PCR plates for automated processing

Requires:

Thermo Scientific KingFisher 96 or KingFisher Flex Instrument or other magnetic separator device

Specifications

• Description: Pierce Titanium Dioxide Magnetic Phosphopeptide Enrichment Kit
• Kit Contents: Washing Buffer, 25mL, Binding Buffer, 100mL, Thermo-Fast 96 Robotic PCR Plates, 0.2mL wells, 2 plates, Elution Buffer, 3mL, TiO2 Magnetic Beads (20X), 1mL
• Quantity: 96 Reaction Kit

• Formulation: Multi-component kit with TiO2 magnetic beads
• Support Type: Magnetic Bead
• Sufficient For: Purifying 96 x 100ug peptide samples