Pig anti-Human IgE Fc Secondary Antibody, Novus Biologicals™

Pig Polyclonal Antibody

Brand:  Bio-Techne NBP2-69114

Product Code. 18105022

Description

IgE Fc Polyclonal specifically detects IgE Fc in Human samples. It is validated for ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunoblotting, Dot Blot.

Specifications

• Antigen: IgE Fc
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: Lyophilized from PBS (pH 7.2) with No Preservative
• Immunogen: Purified homogenous IgE isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Quantity: 10 mg
• Test Specificity: The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with human IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in human serum.
• Target Species: Human
• Form: Lyophilized

• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoblot, Dot Blot
• Concentration: LYOPH
• Dilution: ELISA 1:100-1:2000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting 1:100 - 1:2000, Dot Blot 1:100-1:2000
• Host Species: Porcine
• Purification Method: Ion exchange chromatography
• Primary or Secondary: Secondary
• Reconstitution: Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.