missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

£4.54 - £7240.00

Specifications

GC-Rich PCR Performance High
Polymerase Platinum II Taq Hot-Start DNA Polymerase
Reaction Speed Fast or Standard
Product Type Hot Start DNA Polymerase
Shipping Condition Dry Ice
View More Specs

Products 5
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
15949082
View Documents
Invitrogen™
14966000
25 Reactions
£4.54
Each
Please to purchase this item. Need a web account? Register with us today!
15825066
View Documents Promotion Details
Invitrogen™
14966001
100 Reactions
£112.00
100 reactions
Please to purchase this item. Need a web account? Register with us today!
15836325
View Documents
Invitrogen™
14966005
500 Reactions
£473.00
500 reactions
Please to purchase this item. Need a web account? Register with us today!
15846325
View Documents
Invitrogen™
14966025
2500 Reactions
£2180.00
Each
Please to purchase this item. Need a web account? Register with us today!
17126098
View Documents
Invitrogen™
14966100
4 x 2500 Reactions
£7240.00
10000 reactions
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.

Features of Platinum II Taq Hot-Start DNA Polymerase include:

  • Innovative buffer—enables universal annealing temperature by isostabilizing primer-template duplex structures
  • Engineered Taq DNA polymerase—confers fast cycling and resistance to common inhibitors
  • Platinum hot-start technology—enables superior specificity, sensitivity, and yields; allows for room temperature reaction setup

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic 'hot start' provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Taq Hot-Start DNA Polymerase, different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Platinum II Taq Hot-Start DNA Polymerase is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Use Platinum II Taq Hot-Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR, in applications like genotyping, high-throughput PCR, or with samples of suboptimal purity.

For increased convenience, we offer Platinum II Hot-Start PCR Master Mix (2X) (Cat. No. 14000012), where Platinum II Taq Hot-Start DNA Polymerase is provided in a ready-to-use mixture with Platinum II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. Platinum II Hot-Start Green PCR Master Mix (2X) is also available, which additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels, further streamlining the PCR workflow from setup to final analysis of the result.

Specifications

Specifications

High
Fast or Standard
Dry Ice
1X
Separate Components
Platinum II Taq Hot-Start DNA Polymerase
Hot Start DNA Polymerase
2X
3'-A
5 kb or less
Videos
Safety and Handling

Safety and Handling

missing translation for 'shelfLife' : 24 Months

SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.