Invitrogen™ ProBond™ Nickel-Chelating Resin

A nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence.

Product Code	Brand	Quantity
11500656	Invitrogen™ R80115	150 mL
11590646	Invitrogen™ R80101	50 mL

Description

• ProBond™ Nickel-Chelating Resin is a nickel-charged affinity resin used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
• Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine
• One-step purification can be performed under both native and denaturing conditions
• ProBond™ Resin uses the chelating ligand iminodiacetic acid (IDA) coupled to a highly cross-linked 6% agarose resin
• Suitable for use in FPLC, batch, and gravity flow applications
• Guaranteed stable for 6 months when properly stored at 4°C

Specifications

• Content And Storage: The ProBond™ resin is pre-charged and able to bind 1-5 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 20% ethanol. The resin will appear blue in color when charged with Ni²⁺ . Store at +4°C. ProBond™ resin is guaranteed stable for 6 months when properly stored.
• Product Line: ProBond™
• Form: Suspension

• Stationary Phase: Nickel-Chelating
• Type: Resin

For Research Use Only. Not for use in diagnostic procedures.