Invitrogen™ Progranulin (GRN) Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMGRNX10

Product Code. 18507824

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.

Description

The Mouse Progranulin (GRN) ELISA quantitates Ms Progranulin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Progranulin. Principle of the method The Mouse Progranulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Progranulin is the precursor protein of Granulin also called as Proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature Granulin. Both Granulin and smaller cleavage product of Granulin regulates cell growth. Progranulin stimulates proliferation of many epithelial cells, promotes their anchorage-independent growth and, when overexpressed, confers epithelial tumorigenicity. Progranulin/epithelin gene is multifunctional, with important constitutive roles in epithelial homeostasis, reproductive, immunological, and neuronal function. Progranulin constitutively expressed in a number of epithelia, particularly in the skin, GI tract, and reproductive system. Progranulin is expressed in immune cells in vivo and in specific neurons in the brain.

Specifications

• Accession Number: P28798
• Assay Sensitivity: 20 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Supernatant
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 14824
• Interassay CV: <12%
• Kit Contents: Stop Solution
• Quantity: 10 x 96 Tests
• Research Discipline: Development
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.

• Assay Range: 20.58 to 15,000 pg/mL
• Calibration Range: 20 pg/mL to 15000 pg/mL
• Conjugate: Biotin
• Protein Family: Transcription Factors
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, O x 40, Ligand, P-Cadherin, Periostin, PlGF-2, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon.
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Grn
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 1 µL; Serum, 1 µL; Supernatant, 100 µL
• Synonym: RP23-226P4.4, Pgrn, epithelin
• Test Time: 1 hr. 20 min.