Invitrogen™ Progranulin (GRN) Mouse ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
18507824	Invitrogen™ EMGRNX10	10 x 96 Tests
15445273	Invitrogen™ EMGRN	96 Tests

Description

The Mouse Progranulin (GRN) ELISA quantitates Ms Progranulin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Progranulin. Principle of the method The Mouse Progranulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Progranulin is the precursor protein of Granulin also called as Proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature Granulin. Both Granulin and smaller cleavage product of Granulin regulates cell growth. Progranulin stimulates proliferation of many epithelial cells, promotes their anchorage-independent growth and, when overexpressed, confers epithelial tumorigenicity. Progranulin/epithelin gene is multifunctional, with important constitutive roles in epithelial homeostasis, reproductive, immunological, and neuronal function. Progranulin constitutively expressed in a number of epithelia, particularly in the skin, GI tract, and reproductive system. Progranulin is expressed in immune cells in vivo and in specific neurons in the brain.

Specifications

• Accession Number: P28798
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 14824
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Development
• Synonym: RP23-226P4.4, Pgrn, epithelin
• Test Time: 1 hr. 20 min.

• Assay Sensitivity: 20 pg/mL
• Conjugate: Biotin
• Protein Family: Transcription Factors
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: Grn
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Mouse
• Total Assay Time: 4 hr. 45 min.