Cytiva Protein A and Protein G Mag Sepharose™

Simplify enrichment of target proteins by immunoprecipitation or pull-down applications

Product Code	Brand	Bead Type	Binding Capacity	Ligand	Quantity
10300695	Cytiva 28-9440-06	Protein A Mag Sepharose	8 to 17mg Human IgG/mL	Native Protein A	500 μL
10249574	Cytiva 28-9440-08	Protein G Mag Sepharose	13 to 22mg Human IgG/mL	Protein G	500 μL
10488634	Cytiva 28-9513-79	Protein G Mag Sepharose	13 to 22mg Human IgG/mL	Protein G	4 x 500 μL
10259574	Cytiva 28-9513-78	Protein A Mag Sepharose	8 to 17mg Human IgG/mL	Native Protein A	4 x 500 μL

Description

Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.

There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.

Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

• Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target protein
• Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
• Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
• Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed

Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: Native protein A; Native protein G
Binding capacity Protein A: 8-17mg human IgG/mL gel
Binding capacity Protein G: 13-22mg human IgG/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% ethanol
Storage temperature: 4°C to 8°C

Specifications

• Matrix: Paramagnetic spherical, highly cross linked agarose particles
• Particle Size: 37 to 100 μm
• Content And Storage: 4°C to 8°C

Safety and Handling

Product Identifier

• Protein A Mag Sepharose, 1 x 500 ul

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN AQUATIC-Contains 50% of components with unknown hazards to the aquatic environment.