Cytiva Protein G HP MultiTrap™ Plates

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates

Brand:  Cytiva 28-9031-35

Product Code. 11743329

Includes: Four prepacked Protein G HP MultiTrap 96-well filter plates with instructions, plus a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.

Description

• Flexible format, with protocols for both classic elution and cross-link methods
• Formatted for elution for both electrophoresis and LC-MS analysis workflows
• Fast-binding kinetics and high capacity provide high yield
• Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
• Can be used with centrifugation or vacuum, manually or in automated robotic systems
• Easy scale-up with HiTrap™ Protein G HP prepacked columns

• Matrix: Highly cross-linked agarose, 6%
• Binding capacity: approx. 25mg human IgG/mL medium (0.5 to 1mg IgG/well)
• Average particle size: 34μm
• pH stability: 3 to 9 (long term); 2 to 9 (short term)
• Well volume: 800μL
• Centrifugation force: <700 x G
• Vacuum pressure: -0.5 bar (max.)

Specifications

• Product Type: Protein G Sepharose HP MultiTrap plate
• Includes: 4 Prepacked Protein G HP MultiTrap 96-well filter plates with instructions, plus a proto col
• Ligand Type: Recombinant Protein G lacking Albumin-binding region (2mg/mL medium)
• pH Range: 3 to 9 (long term), 2 to 9 (short term)
• Support Type: 6% Highly Cross-linked Agarose
• Column Type: Protein G Sepharose Plate

• Quantity: 4 Pack
• Material: Polypropylene, Polyethylene
• Max. Pressure: -0.5 bar
• Particle Size: 34μm
• Content And Storage: 2° to 8°C
• For Use With (Application): Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked 96-well filter plates that are ready to use, electrophoresis or LC/MS

Safety and Handling

Product Identifier

• Protein G HP MultiTrap

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol
• UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.