Cytiva Protein G HP SpinTrap™ Columns

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns

Brand:  Cytiva 28-9031-34

Product Code. 11580644

Includes: 16 prepacked Protein G HP SpinTrap columns, one plastic bottom cap removal tool, and instructions. Also included is a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.

Description

• Protocols for both classic elution and cross-link methods; designed for use with a standard microcentrifuge
• Formatted for elution for both electrophoresis and LC-MS analysis workflows
• Fast-binding kinetics and high capacity provide high yield
• Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
• Easy scale-up with HiTrap™ Protein G HP prepacked columns

• Matrix: Highly cross-linked agarose, 6%
• Binding capacity: >1mg human IgG/column
• Average particle size: 34μm
• pH stability: 3 to 9 (long term); 2 to 9 (short term)
• Column volume: 800μL
• Max. sample loading volume: 600μL

Specifications

• Product Type: Protein G Sepharose HP SpinTrap spin column
• Includes: 16 Prepacked Protein G HP SpinTrap column, one Plastic botto m cap removal to ol and instructions, proto col
• pH Range: 3 to 9 (long term), 2 to 9 (short term)
• Support Type: 6% Highly Cross-linked Agarose
• Column Type: Protein G Sepharose Spin Column

• Quantity: 800μL
• Ligand Type: Recombinant Protein G lacking Albumin-binding region (2mg/mL medium)
• Particle Size: 34μm
• Content And Storage: 4° to 8°C
• For Use With (Application): Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked spin column, electrophoresis or LC/MS

Safety and Handling

Product Identifier

• Protein G HP SpinTrap

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol