Macherey-Nagel™ Protino™ Ni-IDA Resin

For Purification of Polyhistidine-Tagged Proteins, Batch Binding, Gravity Flow Column Chromatography, MPLC/FPLC Applications

Product Code	Brand	Quantity
11932532	Macherey-Nagel™ 745210.5	5 g Dry Silica Bulk Resin
11972412	Macherey-Nagel™ 745210.30	30 g Dry Silica Bulk Resin

Description

Protino™ Ni-IDA, the purer alternative for higher yields of His-tag proteins

• Chelating group IDA, three protein binding sites
• High protein yield/recovery even from diluted samples
• High protein concentration
• High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
• Dry resin, storage at room temperature

Protino™ Ni-IDA packed columns

• Ready to use pre-packed columns filled with dry Protino™ Ni-IDA resin
• Buffers included

Protino™ Ni-IDA resin

• Dry bulk matrix
• Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell - highest flexibility
• Batch procedure, gravity flow column chromatography, FPLC™ applications
• Empty Protino™ columns available for use with Protino™ Ni-IDA resin
• Empty 96 well receiver plates available for use with Protino™ Ni-IDA resin

Protino™ Multi-96 Ni-IDA

• Ready to use pre-packed 96 well plates filled with dry Protino™ Ni-IDA resin
• Buffers included For purification of polyhistidine-tagged proteins, batch binding, gravity flow column chromatography, MPLC/FPLC™ applications.

Specifications

• Binding Capacity: 20mg/g
• Kit Contents: Buffers
• Max. Operating Pressure: 145 psi
• Type: Resin

• Binding Property: Batch
• Matrix: Macroporous Silica
• For Use With (Application): For Purification of Polyhistidine-Tagged Proteins, Batch Binding, Gravity Flow Column Chromatography, MPLC/FPLC Applications
• Ligand Type: IDA (iminodiacetic acid)

Safety and Handling

Signal Word

• Danger

Hazard Category

• Skin sensitiser Category 1
• Respiratory sensitiser Category 1
• Germ cell mutagenicity Category 2
• Carcinogenicity Category 1A
• LONG-TERM AQUATIC HAZARD Chronic 1

Hazard Statement

• H317-May cause an allergic skin reaction.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H341-Suspected of causing genetic defects.
• H350i-May cause cancer by inhalation.
• H410-Very toxic to aquatic life with long lasting effects.

Precautionary Statement

• P201-Obtain special instructions before use.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P391-Collect spillage.
• P405-Store locked up.

Supplemental information

• MIXTURE LIST-Contains : resin with Ni in nickel(II) complexes