GeneBLAzer™ RARγ-UAS-bla HEK 293T Cells

GeneBLAzer™ RARγ-UAS-bla HEK 293T Cells contain the ligand binding domain (LBD) of the human retinoic acid receptor gamma.

Brand:  GeneBLAzer™ K1763

Product Code. 10043963

Description

• Alternate Agonists: 4-hydroxytamoxifen, Chlordane, Toxaphene
• Primary Agonist: XCT790
• The GeneBLAzer™ RAR gamma-UAS-bla HEK 293T cells contain the ligand binding domain (LBD) of the human retinoic acid receptor gamma (RAR gamma) fused to the DNA-binding domain of GAL4, stably integrated in the GeneBLAzer™ UAS-bla HEK 293T cell line.
• GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS).
• When an agonist binds to the LBD of the GAL4 (DBD)-RAR gamma (LBD) fusion protein, it binds to the UAS, inducing transcription of beta-lactamase.
• GeneBLAzer™ RAR gamma-UAS-bla HEK 293T cells have been tested for assay performance using variable assay conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time.
• The Z' and EC50 concentrations of all-trans-retinoic acid and other stimuli have been determined. Additional testing data are available upon request. RAR gamma is a member of the retinoic acid receptor family of nuclear receptors. Retinoids, vitamin A and its natural and synthetic analogues, are a very important group of hormones that regulate a wide variety of biological functions including embryogenesis, cell growth, and cell differentiation.
• Retinoids are beneficial in a wide variety of clinical cases including dermatology, oncology, diabetes, and diseases associated with HPV.
• RAR gamma is the predominant RAR subtype present in the skin and it constitutes more than 90% of the total skin or keratinocyte RAR repertoire.
• Skin irritation is a classical toxicity associated with RAR pan-agonists, like RA or TTNPB, and is believed to be a manifestation of RAR gamma activation.
• Furthermore, it has been shown that tumor-specific apoptosis can be driven by RAR gamma selective agonists, for pancreatic cancer cells.

Specifications

• Content And Storage: Liquid Nitrogen
• For Use With (Application): Nuclear Receptors
• Type: Dividing Cells

• Detection Method: Fluorescent
• Quantity: 2 x 10⁶ Cells