Novus Biologicals™ Recombinant Human IL-6 Protein

Recombinant Protein

Brand:  Novus Biologicals™ NBP2-34901-1mg

Product Code. 18472542

Description

Assay #1:

• Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.1 ng/mL, corresponding to a specific activity of >1.0 x 10⁷ IU/mg.

Assay #2:

• Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine T1165 cells is less than 0.8 ng/mL, corresponding to a specific activity of >1.25 x 10⁶ IU/mg.

Specifications

• Accession Number: P05231, P05231, P05231
• For Use With (Application): PAGE, Bioactivity
• Gene ID (Entrez): 3569
• Quantity: 1 mg
• Endotoxin Concentration: Less than 1.0 EU/ug of IL-6 as determined by LAL method.
• Gene Symbol: IL6
• Research Category: Adaptive Immunity, Biologically Active Proteins, Cancer, Cell Cycle and Replication, Cytokine Research, Immunology, Innate Immunity, Neuroscience
• Protein: IL-6

• Concentration: LYOPH
• Formulation: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
• Molecular Weight (g/mol): 20.8 kDa
• Storage Requirements: Store at -20°C to -70°C as supplied. After reconstitution, store at 2°C to 8°C for 1 month and at -20°C to -70°C for long term storage. Avoid repeated freeze-thaw cycles.
• Gene Alias: B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6
• Reconstitution: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20°C.
• Purity or Quality Grade: >95% by SDS-PAGE and HPLC