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enQuireBio™ Recombinant Human MMP-2 Protein

A cDNA sequence encoding the MMP-2 was constructed and used to recombinantly synthesize the protein.

£99.15 - £5160.00

Specifications

Name	MMP-2 Protein
Regulatory Status	Research Use Only
Endotoxin Concentration	< 1.0 EU per ug protein as determined by the LAL method.
Biological Activity	The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001). , The specific activity is > 1,000 pmoles/min/microg. Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA). Activation Protocol:1. Dilute MMP2 to 100microg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5. 2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO. 3. Incubate at 37C for 1 hour.
Product Type	Recombinant Protein

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Product Code	Brand	Quantity	Price	Quantity & Availability
Product Code	Brand	Quantity	Price	Quantity & Availability
15965178 View Documents Promotion Details missing translation for 'viewProductCertificates'	enQuireBio™ QP10790-2ug	2 μg	£99.15 2µg View alternative products Save Up to »		Please sign in to purchase this item. Need a web account? Register with us today!

15945178 View Documents Promotion Details missing translation for 'viewProductCertificates'	enQuireBio™ QP10790-10ug	10 μg	£155.00 10µg View alternative products Save Up to »		Please sign in to purchase this item. Need a web account? Register with us today!

15955178 View Documents Promotion Details missing translation for 'viewProductCertificates'	enQuireBio™ QP10790-1mg	1 mg	£5160.00 1mg View alternative products Save Up to »		Please sign in to purchase this item. Need a web account? Register with us today!

Specifications

Specifications

Name	MMP-2 Protein
Endotoxin Concentration	< 1.0 EU per ug protein as determined by the LAL method.
Product Type	Recombinant Protein
Species	Human (HEK293)
Sequence	MEALMARGAL TGPLRALCLL GCLLSHAAAA PSPIIKFPGD VAPKTDKELA VQYLNTFYGC PKESCNLFVL KDTLKKMQKF FGLPQTGDLD QNTIETMRKP RCGNPDVANY NFFPRKPKWD KNQITYRIIG YTPDLDPETV DDAFARAFQV WSDVTPLRFS RIHDGEADIM INFGRWEHGD GYPFDGKDGL LAHAFAPGTG VGGDSHFDDD ELWTLGEGQV VRVKYGNADG EYCKFPFLFN GKEYNSCTDT GRSDGFLWCS TTYNFEKDGK YGFCPHEALF TMGGNAEGQP CKFPFRFQGT SYDSCTTEGR TDGYRWCGTT EDYDRDKKYG FCPETAMSTV GGNSEGAPCV FPFTFLGNKY ESCTSAGRSD GKMWCATTAN YDDDRKWGFC PDQGYSLFLV AAHEFGHAMG LEHSQDPGAL MAPIYTYTKN FRLSQDDIKG IQELYGASPD IDLGTGPTPT LGPVTPEICK QDIVFDGIAQ IRGEIFFFKD RFIWRTVTPR DKPMGPLLVA TFWPELPEKI DAVYEAPQEE KAVFFAGNEY WIYSASTLER GYPKPLTSLG LPPDVQRVDA AFNWSKNKKT YIFAGDKFWR YNEVKKKMDP GFPKLIADAW NAIPDNLDAV VDLQGGGHSY FFKGAYYLKL ENQSLKSVKF GSIKSDWLGC
Purity or Quality Grade	Greater than 95% as determined by SDS-PAGE.

Regulatory Status	Research Use Only
Biological Activity	The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RND,Catalog # ES001). , The specific activity is > 1,000 pmoles/min/microg. Recombinant Human MMP-2 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA). Activation Protocol:1. Dilute MMP2 to 100microg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5. 2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO. 3. Incubate at 37C for 1 hour.
Cross Reactivity	Human
Protein Tag	His
Buffer	, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5. 2. Activate MMP2 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO. 3. Incubate at

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