Thermo Scientific™ RevertAid Premium Reverse Transcriptase

An advanced RT enzyme lacking RNase H activity developed through in vitro evolution of M-MuLV RT.

Brand:  Thermo Scientific™ EP0732

Product Code. 10795261

Description

RevertAid Premium Reverse Transcriptase was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks Rnase H activity. The engineered enzyme features dramatically improved thermostability, robustness, and an increased synthesis rate compared to wild type M-MuLV RT.

The eliminated Rnase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased up to 60°C for efficient transcription of RNA regions with a high secondary structure, or to improve specificity using gene-specific primers.

Features include thermostability up to 60°C and an increased cDNA synthesis rate compared to M-MuLV RT. RNase H activity has been eliminated by a point mutation in the RNase H domain of M-MuLV RT, ensuring high yields of full length cDNA up to 20kb.

™ patent pending

• Thermostabl—90% active after incubation at 50°C for 60 minutes in a reaction mixture
• Active up to 60°C
• High yields of full-length cDNA as long as 20kb
• Efficien—complete cDNA synthesis in 30 minutes
• Incorporates modified nucleotides
• Developed through in vitro evolution

Recommended for:

Construction of full length cDNA libraries; Real-time RT-PCR; RT-PCR; DNA labeling; Primer extension; First strand cDNA synthesis

Specifications

• Description: RevertAid Premium Reverse Transcriptase™
• For Use With (Application): cDNA Synthesis, RT-PCR, Real Time PCR (qPCR), Nucleic Acid Labeling
• Includes: 1mL 5X RT Buffer

• Quantity: 10,000U
• Thermostability: 90% active after incubation at 50°C for 60 minutes in a reaction mixture

This product or the use of this product is covered by international patent application WO/2009/125006. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.