Thermo Scientific™ RevertAid Premium Reverse Transcriptase

An advanced RT enzyme lacking RNase H activity developed through in vitro evolution of M-MuLV RT.

Product Code	Brand	Quantity
10795261	Thermo Scientific™ EP0732	10,000U
11511525	Thermo Scientific™ EP0733	4 x 10,000U

Description

RevertAid Premium Reverse Transcriptase was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks Rnase H activity. The engineered enzyme features dramatically improved thermostability, robustness, and an increased synthesis rate compared to wild type M-MuLV RT.

The eliminated Rnase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb. Due to its high thermostability, the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA. The reaction temperature can be increased up to 60°C for efficient transcription of RNA regions with a high secondary structure, or to improve specificity using gene-specific primers.

Highlights

Thermostable -90% active after incubation at 50°C for 60 minutes in a reaction mixture

Active up to 60°C

High yields of full-length cDNA as long as 20 kb

Efficient -complete cDNA synthesis in 30 minutes

Incorporates modified nucleotides

Specifications

• Description: RevertAid Premium Reverse Transcriptase™
• Thermostability: 90% active after incubation at 50°C for 60 minutes in a reaction mixture

This product or the use of this product is covered by international patent application WO/2009/125006. The purchase of this product includes a non-transferable license to use this product for the purchaser's internal research. All other commercial uses of this product, including without limitation product use for diagnostic purposes, resale of product in the original or any modified form or product use in providing commercial services require a separate license from Fermentas.